Galileo AMR allows

  1. Browsing of the repository of antibiotic resistance genes and selected mobile elements
  2. Annotation of resistance genes and associated mobile elements in bacterial DNA sequences
  3. Contribution of new resistance genes and mobile elements not yet in the database

Bacteria that cause infections are becoming increasingly resistant to antibiotics. In Gram-negative bacteria much of this resistance is caused by mobile resistance genes. Understanding the genetic elements responsible for spread of resistance genes is important for monitoring and predicting the spread of resistance in these organisms. We have developed a unique system (Attacca) that has allowed creation an archive of relevant elements and identification of these elements in bacterial DNA sequences. The aim of this project is to make our repository of antibiotic resistance genes available to the wider research community, to allow researchers to annotate sequences containing resistance genes and associated mobile elements using our system and to contribute new entries to the repository as these are found.

Getting Started

Bacteria that cause infections are becoming increasingly resistant to antibiotics. In Gram-negative bacteria much of this resistance is caused by mobile resistance genes. Understanding the genetic elements responsible for spread of resistance genes is important for monitoring and predicting the spread of resistance in these organisms. We have developed a unique system (Attacca) that has allowed creation an archive of relevant elements and identification of these elements in bacterial DNA sequences. The aim of this project is to make our repository of antibiotic resistance genes available to the wider research community, to allow researchers to annotate sequences containing resistance genes and associated mobile elements using our system and to contribute new entries to the repository as these are found.

How to Annotate a Sequence

  1. Select “Annotate new sequence” (login required)
  2. Enter a short description/name for the sequence you want to annotate (this is for your own use)
  3. Choose a sequence type from the drop down (this will affect how your sequence is processed)
  4. Choose the type of DNA molecule (chromosome, plasmid) if known (optional)
  5. Enter species information if known (optional)
  6. Paste in your sequence or upload a FASTA file using the Browse button (up to 5MB per file)
  7. Click “Annotate” to start the annotation process
  8. The sequence will appear in your work area under “My sequences”.
  9. You can annotate more sequences without waiting for the annotation to complete.

Sequence Annotation

in progress

When a sequence is first submitted its status will be listed as “in progress”. Annotation time will depend on the length of your sequence, the load on our system and whether a review is required or not. You can now close the browser and return to the “My sequences” page at a later time to check the status of your sequences.