This poster was presented at the Clinical Virology Symposium on May 6th, 2019 by Dr. Julianne Brown, Clinical Scientist in the Virology Department at Great Ormond Street Hospital for Children NHS Foundation Trust in London.

Abstract:

“Viral infections in solid organ and bone marrow transplant recipients can cause outcomes ranging from severe illness to transplant failure and even death. Current diagnostic assays use PCR to measure qualitative and quantitative presence of a target virus enabling clinical assessment of progression and the efficacy of treatment, however, these require multiple reactions and fail to detect non-targeted co-infections, which are common in immunocompromised patients.

Metagenomic next-generation sequencing (mNGS) has the potential to determine the presence and abundance of transplant-related viruses, as well as identify co-infections, in an unbiased manner.”

In this presentation you will learn:

• What is NGS-based metagenomics (mNGS) is and how it can be applied to pathogen detection
• How mNGS compares with and improves upon qPCR as a detection platform
• What the mNGS workflow looks like when deployed in your laboratory
• Why deploying mNGS into your laboratory makes sense