Poster – Evaluation of the Galileo™ Pathogen Solution Next-Generation Sequencing Pipeline for the Identification and Quantification of DNA Viruses in Transplant Patients

Poster – Evaluation of the Galileo™ Pathogen Solution Next-Generation Sequencing Pipeline for the Identification and Quantification of DNA Viruses in Transplant Patients

NGS-based metagenomics in the clinical virology laboratory

Poster Presentation at the Association for Molecular Pathology Conference 2018
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Evaluation of the Galileo™ Pathogen Solution Next-Generation Sequencing Pipeline for the Identification and Quantification of DNA Viruses in Transplant Patients

This poster was presented at the Association for Molecular Pathology Conference, which took place in San Antonio Texas in November 2018 by Dr. Meredith L. Carpenter, Director Of Assay Research & Development at Arc Bio.

Abstract:

“Solid organ and hematopoietic stem cell transplant recipients are uniquely susceptible to infections, often with increased severity, due to a number of common and opportunistic viruses. Specifically, viral infections including cytomegalovirus (CMV), Epstein-Barr virus (EBV), BK virus (BKV), human herpesvirus-6 (HHV6) and human Adenovirus (ADV) can cause outcomes ranging from severe illness to transplant failure and even death.

There are only handful of commercial assays available for detecting/quantifying CMV, with the remainder of assays available as either ASRs or LDTs. These tests measure the presence of and/or viral load of the target virus, enabling clinical assessment of progression and the efficacy of treatment. However, all of these tests are run individually, and they fail to detect non-targeted co-infections, which are common in immunocompromised patients.

Next-generation sequencing (NGS) has the potential to enable clinicians to determine the presence and abundance of transplant-related viruses with unprecedented precision, as well as identify co-infections in an unbiased manner. Sequencing of clinical samples can reveal pathogens that targeted investigations miss, including un-culturable or closely related species, all in a single test.”

In this presentation you will learn:

  • What is NGS-based metagenomics (mNGS) is and how it can be applied to pathogen detection
  • How mNGS compares with and improves upon qPCR as a detection platform
  • What the mNGS workflow looks like when deployed in your laboratory
  • Why deploying mNGS into your laboratory makes sense
  • How Arc Bio has approached mNGS with its Galileo™ platform
For research use only. Not for use in diagnostics or diagnostic procedures.

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