Poster – Megaplasmids on the rise: combining sequencing approaches to fully resolve a carbapenemase – encoding plasmid in a novel Pseudomonas species
Detection of Antimicrobial Resistance
Poster Presentation at the 9th European Congress of Clinical Microbiology & Infectious Diseases (ECCMID 2019) Learn more about Galileo™Megaplasmids on the rise: combining sequencing approaches to fully resolve a carbapenemase – encoding plasmid in a novel Pseudomonas species
This poster was presented at the 29th European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), which took place in Amsterdam in April 2019 by Dr. João Botelho at UCIBIO/REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
Abstract:
“Horizontal transfer of plasmids plays a pivotal role in the dissemination of antibiotic resistance genes and the emergence of multidrug-resistant bacteria. Sequencing of plasmids is thus paramount for the success of accurate epidemiological tracking strategies in the hospital setting and routine surveillance.
Fully resolving plasmids using short-read sequencing technologies remains challenging due to the presence of numerous long repeated regions, and currently, the most accurate approach to assemble these plasmids is to use a combination of short-read and long-read methods.
Here, we combined Nanopore and Illumina sequencing to fully assemble a carbapenemase-encoding megaplasmid carried by an isolate belonging to a putative novel pseudomonas species.”
